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b25909 cas  (MedChemExpress)


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    Structured Review

    MedChemExpress b25909 cas
    B25909 Cas, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b25909+cas/Butylated+hydroxytoluene/pm39392759-260-89-104
    Average 94 stars, based on 8 article reviews
    b25909 cas - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: Gut Bacteroides ovatus ameliorates renal fibrosis by promoting the production of HDCA through upregulation of Clostridium scindens.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Fibronectin Abcam Cat# ab2413; RRID: AB_2262874 Rabbit monoclonal anti-Collagen I Abcam Cat# ab260043; RRID: AB_2922767 Rabbit monoclonal anti-Vimentin (D21H3) Cell Signaling Technology Cat# 5741; RRID: AB_10695459 Rabbit monoclonal anti-alpha smooth muscle Actin Abcam Cat# ab124964; RRID: AB_11129103 Rabbit monoclonal anti-PAI1 Abcam Cat# ab182973; RRID: N/A Rabbit monoclonal anti-TNF alpha Abcam Cat# ab215188; RRID: AB_2935774 Rabbit monoclonal anti-IL-6 Abcam Cat# ab259341; RRID: AB_2927381 Rabbit polyclonal anti-GPCR TGR5 Abcam Cat# ab72608; RRID: AB_2112165 Rabbit polyclonal anti-GLP-1R Proteintech Cat# 26196-1-AP; RRID: AB_2880421 Rabbit polyclonal anti-GLP-1R HUABIO Cat# ER1909-68; RRID: N/A Rabbit monoclonal anti-ZO1 Abcam Cat# ab276131; RRID: AB_3083081 Mouse monoclonal anti-Occludin Proteintech Cat# 66378-1-Ig; RRID: AB_2881755 Rabbit polyclonal anti-CYP3A4 Proteintech Cat# 18227-1-AP; RRID: AB_2090329 Mouse monoclonal anti-b-Actin (8H10D10) Cell Signaling Technology Cat# 3700; RRID: AB_2242334 Mouse monoclonal anti-GAPDH Proteintech Cat# HRP-60004; RRID: AB_2737588 Anti-Rabbit-IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 HRP-conjugated Goat Anti-Mouse-IgG (H + L) Proteintech Cat# SA00001-1; RRID: AB_2722565 Mouse monoclonal anti-Na+/K+ ATPaes a-1 Merk Millipore Cat# 05–369; RRID: AB_309699 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) Proteintech Cat# SA00003-2; RRID: AB_2890897 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Mouse IgG (H + L) Proteintech Cat# SA00003-1; RRID: AB_2890896 Bacterial and virus strains Bacteroides ovatus ATCC ATCC 8483 Clostridium scindens ATCC ATCC 35704 Bacteroides fragilis ATCC ATCC 25285 Clostridium sporogenes JCM JCM 1416 Clostridium thermopalmarium ATCC ATCC 51427 E. coli ATCC ATCC MG1655 rAAV-U6-shRNA (scramble)-CMV-EGFP-SV40 polyA BrainVTA Technology N/A rAAV-U6-shRNA (Glp1r)-CMV-EGFP-SV40 polyA BrainVTA Technology N/A Biological samples Serum from humans This paper N/A Feces from humans This paper N/A Tissues from humans This paper N/A Serum from mice This paper N/A Feces from mice This paper N/A Tissues from mice This paper N/A (Continued on next page) Cell Reports 43, 114830, October 22, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Adenine J&K Scientific Cat# 391047; CAS: 73-24-5 Hyodeoxycholic acid Sigma-Aldrich Cat# H3878 CAS: 83-49-8 Liraglutide Novo Nordisk S20160004 Dulaglutide Vetter Pharma-Fertigung GmbH & Co.KG S20190022 Ketoconazole J&K Scientific Cat# 973125; CAS: 65277-42-1 Neohesperidin YuanYe Biotech Cat# S24578 CAS: 13241-33-3 Ampicillin YuanYe Biotech Cat# S17018 CAS: 69-52-3 Gentamicin YuanYe Biotech Cat# S17024 CAS: 1405-41-0 Neomycin YuanYe Biotech Cat# S17028 CAS: 1405-10-3 Metronidazole YuanYe Biotech Cat# S17079 CAS: 443-48-1 Vancomycin YuanYe Biotech Cat# S17059 CAS: 1404-93-9 2,6-di-tert-butyl-p-cresol (BHT) YuanYe Biotech Cat# B25909 CAS: 128-37-0 AceQ qPCR SYBR Green Master Mix Vazyme Biotech Q141-02 Aristolochic acid A MedChemExpress Cat# HY-N0510 CAS: 313-67-7 Human TGF-b PEPROTECH Cat# 100-21C Lithocholic acid J&K Scientific Cat# 980912; CAS: 434-13-9 Deoxycholic acid J&K Scientific Cat# 393388; CAS: 83-44-3 Chenodeoxycholic acid J&K Scientific Cat# 951898; CAS: 474-25-9 SBI-115 MedChemExpress Cat# HY-111534 CAS: 882366-16-7 HiScript II QRT SuperMix Vazyme Biotech Cat# R233-01 AceQ qPCR SYBR Green Master Mix Vazyme Biotech Cat# Q141-02 Critical commercial assays Mouse LPS ELISA Kit Wuhan Mosak Biotech Cat# KT37561 Mouse GLP-1 ELISA Kit Elabscience Cat# E-EL-M3012 Human GLP-1 ELISA Kit Elabscience Cat# E-EL-H6025 cAMP ELISA Kit Elabscience Cat# E-EL-0056c TIANamp Stool DNA Kit TIANGEN Biotech Cat# DP328 Enhanced BCA Protein Assay Kit Beyotime Cat# P0009 High Pure RNA Isolation Kit Takara Bio N/A Deposited data The raw data from the quantitative analysis of bile acids This paper MTBLS11050 Experimental models: Cell lines Mouse: Primary renal tubular epithelial cells This paper N/A HK-2 cells This paper N/A Caco-2 cells Shanghai FuHeng Biotech Cat# FH0029 (Continued on next page) 18 Cell Reports 43, 114830, October 22, 2024 ..

    Real-time Polymerase Chain Reaction:

    Article Title: Gut Bacteroides ovatus ameliorates renal fibrosis by promoting the production of HDCA through upregulation of Clostridium scindens.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Fibronectin Abcam Cat# ab2413; RRID: AB_2262874 Rabbit monoclonal anti-Collagen I Abcam Cat# ab260043; RRID: AB_2922767 Rabbit monoclonal anti-Vimentin (D21H3) Cell Signaling Technology Cat# 5741; RRID: AB_10695459 Rabbit monoclonal anti-alpha smooth muscle Actin Abcam Cat# ab124964; RRID: AB_11129103 Rabbit monoclonal anti-PAI1 Abcam Cat# ab182973; RRID: N/A Rabbit monoclonal anti-TNF alpha Abcam Cat# ab215188; RRID: AB_2935774 Rabbit monoclonal anti-IL-6 Abcam Cat# ab259341; RRID: AB_2927381 Rabbit polyclonal anti-GPCR TGR5 Abcam Cat# ab72608; RRID: AB_2112165 Rabbit polyclonal anti-GLP-1R Proteintech Cat# 26196-1-AP; RRID: AB_2880421 Rabbit polyclonal anti-GLP-1R HUABIO Cat# ER1909-68; RRID: N/A Rabbit monoclonal anti-ZO1 Abcam Cat# ab276131; RRID: AB_3083081 Mouse monoclonal anti-Occludin Proteintech Cat# 66378-1-Ig; RRID: AB_2881755 Rabbit polyclonal anti-CYP3A4 Proteintech Cat# 18227-1-AP; RRID: AB_2090329 Mouse monoclonal anti-b-Actin (8H10D10) Cell Signaling Technology Cat# 3700; RRID: AB_2242334 Mouse monoclonal anti-GAPDH Proteintech Cat# HRP-60004; RRID: AB_2737588 Anti-Rabbit-IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 HRP-conjugated Goat Anti-Mouse-IgG (H + L) Proteintech Cat# SA00001-1; RRID: AB_2722565 Mouse monoclonal anti-Na+/K+ ATPaes a-1 Merk Millipore Cat# 05–369; RRID: AB_309699 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) Proteintech Cat# SA00003-2; RRID: AB_2890897 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Mouse IgG (H + L) Proteintech Cat# SA00003-1; RRID: AB_2890896 Bacterial and virus strains Bacteroides ovatus ATCC ATCC 8483 Clostridium scindens ATCC ATCC 35704 Bacteroides fragilis ATCC ATCC 25285 Clostridium sporogenes JCM JCM 1416 Clostridium thermopalmarium ATCC ATCC 51427 E. coli ATCC ATCC MG1655 rAAV-U6-shRNA (scramble)-CMV-EGFP-SV40 polyA BrainVTA Technology N/A rAAV-U6-shRNA (Glp1r)-CMV-EGFP-SV40 polyA BrainVTA Technology N/A Biological samples Serum from humans This paper N/A Feces from humans This paper N/A Tissues from humans This paper N/A Serum from mice This paper N/A Feces from mice This paper N/A Tissues from mice This paper N/A (Continued on next page) Cell Reports 43, 114830, October 22, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Adenine J&K Scientific Cat# 391047; CAS: 73-24-5 Hyodeoxycholic acid Sigma-Aldrich Cat# H3878 CAS: 83-49-8 Liraglutide Novo Nordisk S20160004 Dulaglutide Vetter Pharma-Fertigung GmbH & Co.KG S20190022 Ketoconazole J&K Scientific Cat# 973125; CAS: 65277-42-1 Neohesperidin YuanYe Biotech Cat# S24578 CAS: 13241-33-3 Ampicillin YuanYe Biotech Cat# S17018 CAS: 69-52-3 Gentamicin YuanYe Biotech Cat# S17024 CAS: 1405-41-0 Neomycin YuanYe Biotech Cat# S17028 CAS: 1405-10-3 Metronidazole YuanYe Biotech Cat# S17079 CAS: 443-48-1 Vancomycin YuanYe Biotech Cat# S17059 CAS: 1404-93-9 2,6-di-tert-butyl-p-cresol (BHT) YuanYe Biotech Cat# B25909 CAS: 128-37-0 AceQ qPCR SYBR Green Master Mix Vazyme Biotech Q141-02 Aristolochic acid A MedChemExpress Cat# HY-N0510 CAS: 313-67-7 Human TGF-b PEPROTECH Cat# 100-21C Lithocholic acid J&K Scientific Cat# 980912; CAS: 434-13-9 Deoxycholic acid J&K Scientific Cat# 393388; CAS: 83-44-3 Chenodeoxycholic acid J&K Scientific Cat# 951898; CAS: 474-25-9 SBI-115 MedChemExpress Cat# HY-111534 CAS: 882366-16-7 HiScript II QRT SuperMix Vazyme Biotech Cat# R233-01 AceQ qPCR SYBR Green Master Mix Vazyme Biotech Cat# Q141-02 Critical commercial assays Mouse LPS ELISA Kit Wuhan Mosak Biotech Cat# KT37561 Mouse GLP-1 ELISA Kit Elabscience Cat# E-EL-M3012 Human GLP-1 ELISA Kit Elabscience Cat# E-EL-H6025 cAMP ELISA Kit Elabscience Cat# E-EL-0056c TIANamp Stool DNA Kit TIANGEN Biotech Cat# DP328 Enhanced BCA Protein Assay Kit Beyotime Cat# P0009 High Pure RNA Isolation Kit Takara Bio N/A Deposited data The raw data from the quantitative analysis of bile acids This paper MTBLS11050 Experimental models: Cell lines Mouse: Primary renal tubular epithelial cells This paper N/A HK-2 cells This paper N/A Caco-2 cells Shanghai FuHeng Biotech Cat# FH0029 (Continued on next page) 18 Cell Reports 43, 114830, October 22, 2024 ..

    SYBR Green Assay:

    Article Title: Gut Bacteroides ovatus ameliorates renal fibrosis by promoting the production of HDCA through upregulation of Clostridium scindens.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Fibronectin Abcam Cat# ab2413; RRID: AB_2262874 Rabbit monoclonal anti-Collagen I Abcam Cat# ab260043; RRID: AB_2922767 Rabbit monoclonal anti-Vimentin (D21H3) Cell Signaling Technology Cat# 5741; RRID: AB_10695459 Rabbit monoclonal anti-alpha smooth muscle Actin Abcam Cat# ab124964; RRID: AB_11129103 Rabbit monoclonal anti-PAI1 Abcam Cat# ab182973; RRID: N/A Rabbit monoclonal anti-TNF alpha Abcam Cat# ab215188; RRID: AB_2935774 Rabbit monoclonal anti-IL-6 Abcam Cat# ab259341; RRID: AB_2927381 Rabbit polyclonal anti-GPCR TGR5 Abcam Cat# ab72608; RRID: AB_2112165 Rabbit polyclonal anti-GLP-1R Proteintech Cat# 26196-1-AP; RRID: AB_2880421 Rabbit polyclonal anti-GLP-1R HUABIO Cat# ER1909-68; RRID: N/A Rabbit monoclonal anti-ZO1 Abcam Cat# ab276131; RRID: AB_3083081 Mouse monoclonal anti-Occludin Proteintech Cat# 66378-1-Ig; RRID: AB_2881755 Rabbit polyclonal anti-CYP3A4 Proteintech Cat# 18227-1-AP; RRID: AB_2090329 Mouse monoclonal anti-b-Actin (8H10D10) Cell Signaling Technology Cat# 3700; RRID: AB_2242334 Mouse monoclonal anti-GAPDH Proteintech Cat# HRP-60004; RRID: AB_2737588 Anti-Rabbit-IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 HRP-conjugated Goat Anti-Mouse-IgG (H + L) Proteintech Cat# SA00001-1; RRID: AB_2722565 Mouse monoclonal anti-Na+/K+ ATPaes a-1 Merk Millipore Cat# 05–369; RRID: AB_309699 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) Proteintech Cat# SA00003-2; RRID: AB_2890897 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Mouse IgG (H + L) Proteintech Cat# SA00003-1; RRID: AB_2890896 Bacterial and virus strains Bacteroides ovatus ATCC ATCC 8483 Clostridium scindens ATCC ATCC 35704 Bacteroides fragilis ATCC ATCC 25285 Clostridium sporogenes JCM JCM 1416 Clostridium thermopalmarium ATCC ATCC 51427 E. coli ATCC ATCC MG1655 rAAV-U6-shRNA (scramble)-CMV-EGFP-SV40 polyA BrainVTA Technology N/A rAAV-U6-shRNA (Glp1r)-CMV-EGFP-SV40 polyA BrainVTA Technology N/A Biological samples Serum from humans This paper N/A Feces from humans This paper N/A Tissues from humans This paper N/A Serum from mice This paper N/A Feces from mice This paper N/A Tissues from mice This paper N/A (Continued on next page) Cell Reports 43, 114830, October 22, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Adenine J&K Scientific Cat# 391047; CAS: 73-24-5 Hyodeoxycholic acid Sigma-Aldrich Cat# H3878 CAS: 83-49-8 Liraglutide Novo Nordisk S20160004 Dulaglutide Vetter Pharma-Fertigung GmbH & Co.KG S20190022 Ketoconazole J&K Scientific Cat# 973125; CAS: 65277-42-1 Neohesperidin YuanYe Biotech Cat# S24578 CAS: 13241-33-3 Ampicillin YuanYe Biotech Cat# S17018 CAS: 69-52-3 Gentamicin YuanYe Biotech Cat# S17024 CAS: 1405-41-0 Neomycin YuanYe Biotech Cat# S17028 CAS: 1405-10-3 Metronidazole YuanYe Biotech Cat# S17079 CAS: 443-48-1 Vancomycin YuanYe Biotech Cat# S17059 CAS: 1404-93-9 2,6-di-tert-butyl-p-cresol (BHT) YuanYe Biotech Cat# B25909 CAS: 128-37-0 AceQ qPCR SYBR Green Master Mix Vazyme Biotech Q141-02 Aristolochic acid A MedChemExpress Cat# HY-N0510 CAS: 313-67-7 Human TGF-b PEPROTECH Cat# 100-21C Lithocholic acid J&K Scientific Cat# 980912; CAS: 434-13-9 Deoxycholic acid J&K Scientific Cat# 393388; CAS: 83-44-3 Chenodeoxycholic acid J&K Scientific Cat# 951898; CAS: 474-25-9 SBI-115 MedChemExpress Cat# HY-111534 CAS: 882366-16-7 HiScript II QRT SuperMix Vazyme Biotech Cat# R233-01 AceQ qPCR SYBR Green Master Mix Vazyme Biotech Cat# Q141-02 Critical commercial assays Mouse LPS ELISA Kit Wuhan Mosak Biotech Cat# KT37561 Mouse GLP-1 ELISA Kit Elabscience Cat# E-EL-M3012 Human GLP-1 ELISA Kit Elabscience Cat# E-EL-H6025 cAMP ELISA Kit Elabscience Cat# E-EL-0056c TIANamp Stool DNA Kit TIANGEN Biotech Cat# DP328 Enhanced BCA Protein Assay Kit Beyotime Cat# P0009 High Pure RNA Isolation Kit Takara Bio N/A Deposited data The raw data from the quantitative analysis of bile acids This paper MTBLS11050 Experimental models: Cell lines Mouse: Primary renal tubular epithelial cells This paper N/A HK-2 cells This paper N/A Caco-2 cells Shanghai FuHeng Biotech Cat# FH0029 (Continued on next page) 18 Cell Reports 43, 114830, October 22, 2024 ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Gut Bacteroides ovatus ameliorates renal fibrosis by promoting the production of HDCA through upregulation of Clostridium scindens.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Fibronectin Abcam Cat# ab2413; RRID: AB_2262874 Rabbit monoclonal anti-Collagen I Abcam Cat# ab260043; RRID: AB_2922767 Rabbit monoclonal anti-Vimentin (D21H3) Cell Signaling Technology Cat# 5741; RRID: AB_10695459 Rabbit monoclonal anti-alpha smooth muscle Actin Abcam Cat# ab124964; RRID: AB_11129103 Rabbit monoclonal anti-PAI1 Abcam Cat# ab182973; RRID: N/A Rabbit monoclonal anti-TNF alpha Abcam Cat# ab215188; RRID: AB_2935774 Rabbit monoclonal anti-IL-6 Abcam Cat# ab259341; RRID: AB_2927381 Rabbit polyclonal anti-GPCR TGR5 Abcam Cat# ab72608; RRID: AB_2112165 Rabbit polyclonal anti-GLP-1R Proteintech Cat# 26196-1-AP; RRID: AB_2880421 Rabbit polyclonal anti-GLP-1R HUABIO Cat# ER1909-68; RRID: N/A Rabbit monoclonal anti-ZO1 Abcam Cat# ab276131; RRID: AB_3083081 Mouse monoclonal anti-Occludin Proteintech Cat# 66378-1-Ig; RRID: AB_2881755 Rabbit polyclonal anti-CYP3A4 Proteintech Cat# 18227-1-AP; RRID: AB_2090329 Mouse monoclonal anti-b-Actin (8H10D10) Cell Signaling Technology Cat# 3700; RRID: AB_2242334 Mouse monoclonal anti-GAPDH Proteintech Cat# HRP-60004; RRID: AB_2737588 Anti-Rabbit-IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 HRP-conjugated Goat Anti-Mouse-IgG (H + L) Proteintech Cat# SA00001-1; RRID: AB_2722565 Mouse monoclonal anti-Na+/K+ ATPaes a-1 Merk Millipore Cat# 05–369; RRID: AB_309699 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) Proteintech Cat# SA00003-2; RRID: AB_2890897 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Mouse IgG (H + L) Proteintech Cat# SA00003-1; RRID: AB_2890896 Bacterial and virus strains Bacteroides ovatus ATCC ATCC 8483 Clostridium scindens ATCC ATCC 35704 Bacteroides fragilis ATCC ATCC 25285 Clostridium sporogenes JCM JCM 1416 Clostridium thermopalmarium ATCC ATCC 51427 E. coli ATCC ATCC MG1655 rAAV-U6-shRNA (scramble)-CMV-EGFP-SV40 polyA BrainVTA Technology N/A rAAV-U6-shRNA (Glp1r)-CMV-EGFP-SV40 polyA BrainVTA Technology N/A Biological samples Serum from humans This paper N/A Feces from humans This paper N/A Tissues from humans This paper N/A Serum from mice This paper N/A Feces from mice This paper N/A Tissues from mice This paper N/A (Continued on next page) Cell Reports 43, 114830, October 22, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Adenine J&K Scientific Cat# 391047; CAS: 73-24-5 Hyodeoxycholic acid Sigma-Aldrich Cat# H3878 CAS: 83-49-8 Liraglutide Novo Nordisk S20160004 Dulaglutide Vetter Pharma-Fertigung GmbH & Co.KG S20190022 Ketoconazole J&K Scientific Cat# 973125; CAS: 65277-42-1 Neohesperidin YuanYe Biotech Cat# S24578 CAS: 13241-33-3 Ampicillin YuanYe Biotech Cat# S17018 CAS: 69-52-3 Gentamicin YuanYe Biotech Cat# S17024 CAS: 1405-41-0 Neomycin YuanYe Biotech Cat# S17028 CAS: 1405-10-3 Metronidazole YuanYe Biotech Cat# S17079 CAS: 443-48-1 Vancomycin YuanYe Biotech Cat# S17059 CAS: 1404-93-9 2,6-di-tert-butyl-p-cresol (BHT) YuanYe Biotech Cat# B25909 CAS: 128-37-0 AceQ qPCR SYBR Green Master Mix Vazyme Biotech Q141-02 Aristolochic acid A MedChemExpress Cat# HY-N0510 CAS: 313-67-7 Human TGF-b PEPROTECH Cat# 100-21C Lithocholic acid J&K Scientific Cat# 980912; CAS: 434-13-9 Deoxycholic acid J&K Scientific Cat# 393388; CAS: 83-44-3 Chenodeoxycholic acid J&K Scientific Cat# 951898; CAS: 474-25-9 SBI-115 MedChemExpress Cat# HY-111534 CAS: 882366-16-7 HiScript II QRT SuperMix Vazyme Biotech Cat# R233-01 AceQ qPCR SYBR Green Master Mix Vazyme Biotech Cat# Q141-02 Critical commercial assays Mouse LPS ELISA Kit Wuhan Mosak Biotech Cat# KT37561 Mouse GLP-1 ELISA Kit Elabscience Cat# E-EL-M3012 Human GLP-1 ELISA Kit Elabscience Cat# E-EL-H6025 cAMP ELISA Kit Elabscience Cat# E-EL-0056c TIANamp Stool DNA Kit TIANGEN Biotech Cat# DP328 Enhanced BCA Protein Assay Kit Beyotime Cat# P0009 High Pure RNA Isolation Kit Takara Bio N/A Deposited data The raw data from the quantitative analysis of bile acids This paper MTBLS11050 Experimental models: Cell lines Mouse: Primary renal tubular epithelial cells This paper N/A HK-2 cells This paper N/A Caco-2 cells Shanghai FuHeng Biotech Cat# FH0029 (Continued on next page) 18 Cell Reports 43, 114830, October 22, 2024 ..

    Bicinchoninic Acid Protein Assay:

    Article Title: Gut Bacteroides ovatus ameliorates renal fibrosis by promoting the production of HDCA through upregulation of Clostridium scindens.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Fibronectin Abcam Cat# ab2413; RRID: AB_2262874 Rabbit monoclonal anti-Collagen I Abcam Cat# ab260043; RRID: AB_2922767 Rabbit monoclonal anti-Vimentin (D21H3) Cell Signaling Technology Cat# 5741; RRID: AB_10695459 Rabbit monoclonal anti-alpha smooth muscle Actin Abcam Cat# ab124964; RRID: AB_11129103 Rabbit monoclonal anti-PAI1 Abcam Cat# ab182973; RRID: N/A Rabbit monoclonal anti-TNF alpha Abcam Cat# ab215188; RRID: AB_2935774 Rabbit monoclonal anti-IL-6 Abcam Cat# ab259341; RRID: AB_2927381 Rabbit polyclonal anti-GPCR TGR5 Abcam Cat# ab72608; RRID: AB_2112165 Rabbit polyclonal anti-GLP-1R Proteintech Cat# 26196-1-AP; RRID: AB_2880421 Rabbit polyclonal anti-GLP-1R HUABIO Cat# ER1909-68; RRID: N/A Rabbit monoclonal anti-ZO1 Abcam Cat# ab276131; RRID: AB_3083081 Mouse monoclonal anti-Occludin Proteintech Cat# 66378-1-Ig; RRID: AB_2881755 Rabbit polyclonal anti-CYP3A4 Proteintech Cat# 18227-1-AP; RRID: AB_2090329 Mouse monoclonal anti-b-Actin (8H10D10) Cell Signaling Technology Cat# 3700; RRID: AB_2242334 Mouse monoclonal anti-GAPDH Proteintech Cat# HRP-60004; RRID: AB_2737588 Anti-Rabbit-IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 HRP-conjugated Goat Anti-Mouse-IgG (H + L) Proteintech Cat# SA00001-1; RRID: AB_2722565 Mouse monoclonal anti-Na+/K+ ATPaes a-1 Merk Millipore Cat# 05–369; RRID: AB_309699 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) Proteintech Cat# SA00003-2; RRID: AB_2890897 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Mouse IgG (H + L) Proteintech Cat# SA00003-1; RRID: AB_2890896 Bacterial and virus strains Bacteroides ovatus ATCC ATCC 8483 Clostridium scindens ATCC ATCC 35704 Bacteroides fragilis ATCC ATCC 25285 Clostridium sporogenes JCM JCM 1416 Clostridium thermopalmarium ATCC ATCC 51427 E. coli ATCC ATCC MG1655 rAAV-U6-shRNA (scramble)-CMV-EGFP-SV40 polyA BrainVTA Technology N/A rAAV-U6-shRNA (Glp1r)-CMV-EGFP-SV40 polyA BrainVTA Technology N/A Biological samples Serum from humans This paper N/A Feces from humans This paper N/A Tissues from humans This paper N/A Serum from mice This paper N/A Feces from mice This paper N/A Tissues from mice This paper N/A (Continued on next page) Cell Reports 43, 114830, October 22, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Adenine J&K Scientific Cat# 391047; CAS: 73-24-5 Hyodeoxycholic acid Sigma-Aldrich Cat# H3878 CAS: 83-49-8 Liraglutide Novo Nordisk S20160004 Dulaglutide Vetter Pharma-Fertigung GmbH & Co.KG S20190022 Ketoconazole J&K Scientific Cat# 973125; CAS: 65277-42-1 Neohesperidin YuanYe Biotech Cat# S24578 CAS: 13241-33-3 Ampicillin YuanYe Biotech Cat# S17018 CAS: 69-52-3 Gentamicin YuanYe Biotech Cat# S17024 CAS: 1405-41-0 Neomycin YuanYe Biotech Cat# S17028 CAS: 1405-10-3 Metronidazole YuanYe Biotech Cat# S17079 CAS: 443-48-1 Vancomycin YuanYe Biotech Cat# S17059 CAS: 1404-93-9 2,6-di-tert-butyl-p-cresol (BHT) YuanYe Biotech Cat# B25909 CAS: 128-37-0 AceQ qPCR SYBR Green Master Mix Vazyme Biotech Q141-02 Aristolochic acid A MedChemExpress Cat# HY-N0510 CAS: 313-67-7 Human TGF-b PEPROTECH Cat# 100-21C Lithocholic acid J&K Scientific Cat# 980912; CAS: 434-13-9 Deoxycholic acid J&K Scientific Cat# 393388; CAS: 83-44-3 Chenodeoxycholic acid J&K Scientific Cat# 951898; CAS: 474-25-9 SBI-115 MedChemExpress Cat# HY-111534 CAS: 882366-16-7 HiScript II QRT SuperMix Vazyme Biotech Cat# R233-01 AceQ qPCR SYBR Green Master Mix Vazyme Biotech Cat# Q141-02 Critical commercial assays Mouse LPS ELISA Kit Wuhan Mosak Biotech Cat# KT37561 Mouse GLP-1 ELISA Kit Elabscience Cat# E-EL-M3012 Human GLP-1 ELISA Kit Elabscience Cat# E-EL-H6025 cAMP ELISA Kit Elabscience Cat# E-EL-0056c TIANamp Stool DNA Kit TIANGEN Biotech Cat# DP328 Enhanced BCA Protein Assay Kit Beyotime Cat# P0009 High Pure RNA Isolation Kit Takara Bio N/A Deposited data The raw data from the quantitative analysis of bile acids This paper MTBLS11050 Experimental models: Cell lines Mouse: Primary renal tubular epithelial cells This paper N/A HK-2 cells This paper N/A Caco-2 cells Shanghai FuHeng Biotech Cat# FH0029 (Continued on next page) 18 Cell Reports 43, 114830, October 22, 2024 ..

    Isolation:

    Article Title: Gut Bacteroides ovatus ameliorates renal fibrosis by promoting the production of HDCA through upregulation of Clostridium scindens.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Fibronectin Abcam Cat# ab2413; RRID: AB_2262874 Rabbit monoclonal anti-Collagen I Abcam Cat# ab260043; RRID: AB_2922767 Rabbit monoclonal anti-Vimentin (D21H3) Cell Signaling Technology Cat# 5741; RRID: AB_10695459 Rabbit monoclonal anti-alpha smooth muscle Actin Abcam Cat# ab124964; RRID: AB_11129103 Rabbit monoclonal anti-PAI1 Abcam Cat# ab182973; RRID: N/A Rabbit monoclonal anti-TNF alpha Abcam Cat# ab215188; RRID: AB_2935774 Rabbit monoclonal anti-IL-6 Abcam Cat# ab259341; RRID: AB_2927381 Rabbit polyclonal anti-GPCR TGR5 Abcam Cat# ab72608; RRID: AB_2112165 Rabbit polyclonal anti-GLP-1R Proteintech Cat# 26196-1-AP; RRID: AB_2880421 Rabbit polyclonal anti-GLP-1R HUABIO Cat# ER1909-68; RRID: N/A Rabbit monoclonal anti-ZO1 Abcam Cat# ab276131; RRID: AB_3083081 Mouse monoclonal anti-Occludin Proteintech Cat# 66378-1-Ig; RRID: AB_2881755 Rabbit polyclonal anti-CYP3A4 Proteintech Cat# 18227-1-AP; RRID: AB_2090329 Mouse monoclonal anti-b-Actin (8H10D10) Cell Signaling Technology Cat# 3700; RRID: AB_2242334 Mouse monoclonal anti-GAPDH Proteintech Cat# HRP-60004; RRID: AB_2737588 Anti-Rabbit-IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 HRP-conjugated Goat Anti-Mouse-IgG (H + L) Proteintech Cat# SA00001-1; RRID: AB_2722565 Mouse monoclonal anti-Na+/K+ ATPaes a-1 Merk Millipore Cat# 05–369; RRID: AB_309699 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) Proteintech Cat# SA00003-2; RRID: AB_2890897 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Mouse IgG (H + L) Proteintech Cat# SA00003-1; RRID: AB_2890896 Bacterial and virus strains Bacteroides ovatus ATCC ATCC 8483 Clostridium scindens ATCC ATCC 35704 Bacteroides fragilis ATCC ATCC 25285 Clostridium sporogenes JCM JCM 1416 Clostridium thermopalmarium ATCC ATCC 51427 E. coli ATCC ATCC MG1655 rAAV-U6-shRNA (scramble)-CMV-EGFP-SV40 polyA BrainVTA Technology N/A rAAV-U6-shRNA (Glp1r)-CMV-EGFP-SV40 polyA BrainVTA Technology N/A Biological samples Serum from humans This paper N/A Feces from humans This paper N/A Tissues from humans This paper N/A Serum from mice This paper N/A Feces from mice This paper N/A Tissues from mice This paper N/A (Continued on next page) Cell Reports 43, 114830, October 22, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Adenine J&K Scientific Cat# 391047; CAS: 73-24-5 Hyodeoxycholic acid Sigma-Aldrich Cat# H3878 CAS: 83-49-8 Liraglutide Novo Nordisk S20160004 Dulaglutide Vetter Pharma-Fertigung GmbH & Co.KG S20190022 Ketoconazole J&K Scientific Cat# 973125; CAS: 65277-42-1 Neohesperidin YuanYe Biotech Cat# S24578 CAS: 13241-33-3 Ampicillin YuanYe Biotech Cat# S17018 CAS: 69-52-3 Gentamicin YuanYe Biotech Cat# S17024 CAS: 1405-41-0 Neomycin YuanYe Biotech Cat# S17028 CAS: 1405-10-3 Metronidazole YuanYe Biotech Cat# S17079 CAS: 443-48-1 Vancomycin YuanYe Biotech Cat# S17059 CAS: 1404-93-9 2,6-di-tert-butyl-p-cresol (BHT) YuanYe Biotech Cat# B25909 CAS: 128-37-0 AceQ qPCR SYBR Green Master Mix Vazyme Biotech Q141-02 Aristolochic acid A MedChemExpress Cat# HY-N0510 CAS: 313-67-7 Human TGF-b PEPROTECH Cat# 100-21C Lithocholic acid J&K Scientific Cat# 980912; CAS: 434-13-9 Deoxycholic acid J&K Scientific Cat# 393388; CAS: 83-44-3 Chenodeoxycholic acid J&K Scientific Cat# 951898; CAS: 474-25-9 SBI-115 MedChemExpress Cat# HY-111534 CAS: 882366-16-7 HiScript II QRT SuperMix Vazyme Biotech Cat# R233-01 AceQ qPCR SYBR Green Master Mix Vazyme Biotech Cat# Q141-02 Critical commercial assays Mouse LPS ELISA Kit Wuhan Mosak Biotech Cat# KT37561 Mouse GLP-1 ELISA Kit Elabscience Cat# E-EL-M3012 Human GLP-1 ELISA Kit Elabscience Cat# E-EL-H6025 cAMP ELISA Kit Elabscience Cat# E-EL-0056c TIANamp Stool DNA Kit TIANGEN Biotech Cat# DP328 Enhanced BCA Protein Assay Kit Beyotime Cat# P0009 High Pure RNA Isolation Kit Takara Bio N/A Deposited data The raw data from the quantitative analysis of bile acids This paper MTBLS11050 Experimental models: Cell lines Mouse: Primary renal tubular epithelial cells This paper N/A HK-2 cells This paper N/A Caco-2 cells Shanghai FuHeng Biotech Cat# FH0029 (Continued on next page) 18 Cell Reports 43, 114830, October 22, 2024 ..



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